The ThermoMPNN API provides an interface to the AI based ThermoMPNN protocol for performing a full site saturation mutagenesis (SSM) DDG analysis of a protein. A full SSM is generated even if you are only interested in 1 mutation as the entire protocol runs on the order of seconds vs the 10s of minutes per mutation of the older Cartesian DDG tool.

Quickstart

Perform a full SSM

Inputs

The pdbfile containing the structure you would like to perform the SSM on.

Options

there are no options for this protocol currently in use.

Outputs

• predicted_ddgs.csv (csv file)

  • A csv file containing the DDG predictions for every mutation.