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  • Optional - multi-mutations-file

    • TSV file containing mutations to run simultaneously. Similar format to the mutations file, but with each run’s mutations separated with ---

Code Block
---
position	    original_residue	mutation
1	M	DY    mutation
5   K   S
42  T   I
---
position	    original_residue	mutation
1	M	DN    mutation
5   K   S
42  T   K
---
  • Optional - all-mutations mode

    • you can omit an input mutations-file and instead run in all-mutations mode, where all mutations at each position will be selected

  • Optional - dump-pdb mode

    • run with this flag to output the PDB for each mutation as well as the score

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